anti p21 af647 (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
anti p21 af647
Anti P21 Af647, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 5650 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p21+af647/p21/bio_rxiv__2024__12__01__626220-208-20-23
Average 96 stars, based on 5650 article reviews
Anti P21 Af647, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 5650 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p21+af647/p21/bio_rxiv__2024__12__01__626220-208-20-23
Average 96 stars, based on 5650 article reviews
anti p21 af647 - by Bioz Stars,
2026-10
96/100 stars
Images
1) Product Images from "RNA-binding proteins control the G2-M checkpoint of the germinal centre B cell"
Article Title: RNA-binding proteins control the G2-M checkpoint of the germinal centre B cell
Journal: bioRxiv
doi: 10.1101/2024.12.01.626220
Figure Legend Snippet: B cells isolated from LNs of Zfp36l1 fl/fl Zfp36l2 fl/fl CD23 cre mice or Zfp36l1 fl/fl Zfp36l2 fl/fl control mice were co-cultured for 7 days in the presence of CD40LB-expressing feeder cells. a) BrdU and 7AAD staining of CD95 + CD138 − GC-like B cells. b) Venn diagram for genes within the Reactome “Cell cycle” gene set, showing the overlap between those that are increased in Zfp36l1 fl/fl Zfp36l2 fl/fl CD23 cre GC-like B cells compared to Zfp36l1 fl/fl Zfp36l2 fl/fl control cells, bound by mCherry-ZFP36L1 in their 3’UTR, or contain two UAUU motifs separated by up to three nucleotides within their 3’UTR. c) Left: schematic representation of cyclins and CDKs regulating cell cycle progression; iCLIP targets in yellow. Right: Log2 fold change (KO/control) of mRNA encoding cyclins and CDKs as measured by RNA-seq. In yellow iCLIP targets encompassing UAUUN{0-3}UAUU motif within their 3’UTR; in grey mRNAs containing AU-rich binding motifs but not identified as iCLIP targets; in black mRNA without UAUUN{0-3}UAUU binding motif. d, e, g) iCLIP data from GC-like B cells (d7) showing reads across Ccnb1 (d) , Cdk1 (e) , Cdkn1a (g) transcripts with an expanded view of the 3’UTR. f) Histograms depict protein expression of indicated cell-cycle related genes. n= 3 mice per group; data representative of at least two independently performed experiments. h) Left: graph displays normalized counts (mean±SD) of Cdkn1a as measured by RNAseq. Right: protein expression as measured by flow cytometry. n= 3 mice per group.
Techniques Used: Isolation, Control, Cell Culture, Expressing, Staining, RNA Sequencing, Binding Assay, Flow Cytometry
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